8Q2Q

Crystal structure of YTHDC1 in complex with Compound 2b (YL_32)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-25
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	39.980, 103.410, 42.390
Unit cell angles	90.00, 106.00, 90.00

Refinement procedure

Resolution	40.750 - 1.300
R-factor	0.2163
Rwork	0.216
R-free	0.22730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.944
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.750	1.380
High resolution limit [Å]	1.300	1.300
Number of reflections	81179	12993
<I/σ(I)>	15.18
Completeness [%]	99.3
Redundancy	3.35
CC(1/2)	0.999	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5