8Q2P

Structure of the membrane integral lipoprotein N-acyltransferase Lnt from E. coli by using Se-MAG for the the lipid cubic phase crystallization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97939
Spacegroup name	P 21 21 2
Unit cell lengths	156.610, 48.790, 75.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.020 - 1.900
R-factor	0.1863
Rwork	0.184
R-free	0.22360
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.821
Data reduction software	XDS (VERSION Feb 5, 2021 BUILT=20210323)
Data scaling software	XSCALE (VERSION Feb 5, 2021 BUILT=20210323)
Phasing software	SHELXCD (Version 2016/1)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.020	1.970
High resolution limit [Å]	1.900	1.900
Rmeas	0.310	2.950
Number of reflections	87726	6484
<I/σ(I)>	19.4	0.88
Completeness [%]	99.0	99
Redundancy	80.88	4.28
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	293	0.1 M MES-NaOH pH 6.0, 8 %(v/v) MPD, and 0.4 M sodium or potassium thiocyanate