8Q2P
Structure of the membrane integral lipoprotein N-acyltransferase Lnt from E. coli by using Se-MAG for the the lipid cubic phase crystallization
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97939 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 156.610, 48.790, 75.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.020 - 1.900 |
R-factor | 0.1863 |
Rwork | 0.184 |
R-free | 0.22360 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.821 |
Data reduction software | XDS (VERSION Feb 5, 2021 BUILT=20210323) |
Data scaling software | XSCALE (VERSION Feb 5, 2021 BUILT=20210323) |
Phasing software | SHELXCD (Version 2016/1) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.020 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmeas | 0.310 | 2.950 |
Number of reflections | 87726 | 6484 |
<I/σ(I)> | 19.4 | 0.88 |
Completeness [%] | 99.0 | 99 |
Redundancy | 80.88 | 4.28 |
CC(1/2) | 0.990 | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6 | 293 | 0.1 M MES-NaOH pH 6.0, 8 %(v/v) MPD, and 0.4 M sodium or potassium thiocyanate |