8Q2D
Crystal structure of the E. coli PqiC Lipoprotein residues 17-187
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-27 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 61 |
Unit cell lengths | 133.603, 133.603, 65.715 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.730 - 2.080 |
R-factor | 0.2049 |
Rwork | 0.204 |
R-free | 0.22930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.800 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.730 | 2.150 |
High resolution limit [Å] | 2.080 | 2.080 |
Number of reflections | 67192 | 450 |
<I/σ(I)> | 18.79 | |
Completeness [%] | 84.0 | |
Redundancy | 2 | |
CC(1/2) | 0.999 | 0.613 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 0.1 M sodium chloride, 0.1 M MES; pH 6.5, 33% v/v PEG 400, 4% v/v ethylene glycol |