8Q2C

Crystal structure of the E. coli PqiC Lipoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-30
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9797
Spacegroup name	I 4 2 2
Unit cell lengths	132.148, 132.148, 185.043
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.770 - 3.210
R-factor	0.2532
Rwork	0.251
R-free	0.30630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.490
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.770	3.330
High resolution limit [Å]	3.210	3.210
Number of reflections	8788	132
<I/σ(I)>	6.86
Completeness [%]	90.6
Redundancy	2
CC(1/2)	0.977	0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	3 M lithium sulphate, 0.1 M MES; pH 6.5, 25% v/v PEG 400