8Q1K

Structural analysis of PLD3 reveals insights into the mechanism of lysosomal 5' exonuclease-mediated nucleic acid degradation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	60.251, 115.304, 101.082
Unit cell angles	90.00, 106.64, 90.00

Refinement procedure

Resolution	44.650 - 1.510
R-factor	0.15423
Rwork	0.154
R-free	0.18632
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.297
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	HKL2Map
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.650	1.610
High resolution limit [Å]	1.510	1.510
Rmeas	0.007	0.228
Number of reflections	200406	31270
<I/σ(I)>	10.71
Completeness [%]	97.8	94.7
Redundancy	3.77
CC(1/2)	0.990	0.260

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	17% PEG 4000, 0.02 M NaI, 0.6 M Ammonium sulfate