8Q1G

LSD1-CoREST bound to Acetylated K14 of Histone H3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.965459
Spacegroup name	I 2 2 2
Unit cell lengths	119.508, 179.693, 232.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.730 - 2.600
R-factor	0.2227
Rwork	0.222
R-free	0.25510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.201
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.730	2.650
High resolution limit [Å]	2.600	2.600
Rmerge	0.100	2.926
Rmeas	0.114	3.372
Rpim	0.052	1.621
Total number of observations	338452	18223
Number of reflections	74979	4320
<I/σ(I)>	8.8	0.5
Completeness [%]	98.0
Redundancy	4.5	4.2
CC(1/2)	0.996	0.138

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	Sodium Tartrate 1.2M, ADA 0.1M,