8Q1F
D10N,P146A variant of beta-phosphoglucomutase from Lactococcus lactis in complex with native beta-glucose 1,6-bisphosphate intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-23 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.023, 79.663, 79.848 |
| Unit cell angles | 90.00, 97.61, 90.00 |
Refinement procedure
| Resolution | 39.830 - 1.010 |
| Rwork | 0.115 |
| R-free | 0.13480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.486 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.830 | 1.030 |
| High resolution limit [Å] | 1.010 | 1.010 |
| Rmerge | 0.038 | 0.494 |
| Rpim | 0.016 | 0.386 |
| Number of reflections | 166355 | 1151 |
| <I/σ(I)> | 26 | 0.8 |
| Completeness [%] | 80.3 | |
| Redundancy | 6.3 | 1.6 |
| CC(1/2) | 0.999 | 0.743 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 290 | 0.6 mM bPGM-D10N,P146A 50 mM MgCl2 10 mM bG16BP 28% PEG 4000 100 mM sodium acetate 100 mM tris |
