8Q1E
D10N,P146A variant of beta-phosphoglucomutase from Lactococcus lactis in complex with fructose 1,6-bisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-05 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9227 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.120, 83.757, 38.890 |
| Unit cell angles | 90.00, 110.66, 90.00 |
Refinement procedure
| Resolution | 41.913 - 1.230 |
| Rwork | 0.152 |
| R-free | 0.19420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.185 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.760 | 1.250 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.074 | |
| Rpim | 0.031 | 0.953 |
| Number of reflections | 55719 | 2694 |
| <I/σ(I)> | 12.3 | 0.4 |
| Completeness [%] | 100.0 | 98 |
| Redundancy | 6.8 | 5.9 |
| CC(1/2) | 0.999 | 0.315 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 290 | 0.6 mM bPGM-D10N,P146A 50 mM MgCl2 50 mM F16BP 32 % PEG 4000 100 mM sodium acetate 100 mM tris |
