8Q1C
Substrate-free D10N,P146A variant of beta-phosphoglucomutase from Lactococcus lactis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-28 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.650, 117.251, 53.250 |
| Unit cell angles | 90.00, 98.64, 90.00 |
Refinement procedure
| Resolution | 52.702 - 1.679 |
| Rwork | 0.211 |
| R-free | 0.27020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.671 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 117.250 | 1.710 |
| High resolution limit [Å] | 1.679 | 1.680 |
| Rmerge | 0.089 | |
| Rpim | 0.040 | |
| Number of reflections | 52374 | 2284 |
| <I/σ(I)> | 13.8 | 0.6 |
| Completeness [%] | 98.3 | 86.9 |
| Redundancy | 6.5 | 5.7 |
| CC(1/2) | 0.994 | 0.277 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.6 mM bPGM-D10N,P146A 5 mM MgCl2 3 mM AlCl3 20 mM NaF 15 mM glucose 6-phosphate 32 % PEG 4000 200 mM sodium acetate 200 mM tris |
