8Q0P

Crystal Structure of an N-terminal Domain of Variant Surface Glycoprotein 21 (VSG21) of Trypanosome brucei brucei Lister 427

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	174.329, 84.422, 120.095
Unit cell angles	90.00, 100.89, 90.00

Refinement procedure

Resolution	48.530 - 2.010
R-factor	0.1809
Rwork	0.179
R-free	0.21940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.664
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.530	2.082
High resolution limit [Å]	2.010	2.010
Number of reflections	111578	10775
<I/σ(I)>	8.17
Completeness [%]	97.8	95.6
Redundancy	7
CC(1/2)	0.996	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	295	100 mM Tris/HCl, 200 mM CaCL2, 20 % (W/V) PEG 3350