8Q0P
Crystal Structure of an N-terminal Domain of Variant Surface Glycoprotein 21 (VSG21) of Trypanosome brucei brucei Lister 427
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 174.329, 84.422, 120.095 |
Unit cell angles | 90.00, 100.89, 90.00 |
Refinement procedure
Resolution | 48.530 - 2.010 |
R-factor | 0.1809 |
Rwork | 0.179 |
R-free | 0.21940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.664 |
Data reduction software | XDS |
Data scaling software | pointless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.530 | 2.082 |
High resolution limit [Å] | 2.010 | 2.010 |
Number of reflections | 111578 | 10775 |
<I/σ(I)> | 8.17 | |
Completeness [%] | 97.8 | 95.6 |
Redundancy | 7 | |
CC(1/2) | 0.996 | 0.530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 295 | 100 mM Tris/HCl, 200 mM CaCL2, 20 % (W/V) PEG 3350 |