8Q06
EF-hand of MINDY3 Deubiquitylase in Complex with UBL of RAD23A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-08 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.279, 134.139, 45.498 |
| Unit cell angles | 90.00, 114.67, 90.00 |
Refinement procedure
| Resolution | 34.780 - 1.900 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.830 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.780 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.056 | 0.642 |
| Rmeas | 0.060 | 0.699 |
| Rpim | 0.023 | 0.273 |
| Number of reflections | 32714 | 3242 |
| <I/σ(I)> | 19.63 | |
| Completeness [%] | 99.9 | 99.48 |
| Redundancy | 6.9 | 6.3 |
| CC(1/2) | 0.999 | 0.919 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M potassium chloride, 2.2 M ammonium sulphate |
