8Q04
Chlorella sorokiniana Rubisco: D4 symmetry imposed
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.39 |
| Number of Particle | 237035 |
| Software | RELION,PHENIX,EPU,CTFFIND,UCSF Chimera,Coot |
| Symmetry | D4 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
