8PZ4
Structure of alginate transporter, AlgE, solved at wavelength 2.755 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-08-08 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 2.755 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.350, 74.690, 115.900 |
| Unit cell angles | 90.00, 102.48, 90.00 |
Refinement procedure
| Resolution | 56.580 - 1.770 |
| R-factor | 0.1897 |
| Rwork | 0.188 |
| R-free | 0.23390 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.974 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.580 | 1.833 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 88023 | 4019 |
| <I/σ(I)> | 27.32 | |
| Completeness [%] | 96.8 | |
| Redundancy | 26.7 | |
| CC(1/2) | 0.999 | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7.5 | 293 | 0.1 M TRIS-HCL, PH 7.5, 0.1 M SODIUM CITRATE, 18 %(W/V) PEG 400. PROTEIN WAS MIXED WITH 7.8 MAG AT RATIO 1:1 (W:W) TO FORM CUBIC PHASE. |
