8PYH
Crystal structure of the Orange Carotenoid Protein 2 (OCP2) from Crinalium epipsammum PCC 9333
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-14 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.054, 103.727, 109.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.200 - 2.200 |
| R-factor | 0.17991 |
| Rwork | 0.177 |
| R-free | 0.22693 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.048 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.200 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.130 | 0.384 |
| Rmeas | 0.140 | 0.412 |
| Rpim | 0.051 | 0.146 |
| Total number of observations | 265057 | 23129 |
| Number of reflections | 35514 | 2978 |
| <I/σ(I)> | 12.3 | 5.2 |
| Completeness [%] | 99.7 | |
| Redundancy | 7.5 | 7.8 |
| CC(1/2) | 0.993 | 0.874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | 0.1 M Imidazole pH 6.5, 1.0 M Sodium acetate trihydrate |
