8PY0
Sensor domain of Oscillibacter ruminantium chemoreceptor in complex with formate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-15 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 58.379, 58.379, 156.462 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.560 - 1.750 |
| R-factor | 0.1897 |
| Rwork | 0.188 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.697 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.560 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.183 | 1.444 |
| Rmeas | 0.205 | 1.612 |
| Rpim | 0.092 | 0.706 |
| Total number of observations | 150638 | 8664 |
| Number of reflections | 31411 | 1719 |
| <I/σ(I)> | 5.9 | 1.7 |
| Completeness [%] | 97.8 | |
| Redundancy | 4.8 | 5 |
| CC(1/2) | 0.990 | 0.572 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.2 | 293 | 10% (w/v) PEG 8000, 100 mM Potassium phosphate monobasic/ Sodium phosphate dibasic pH 6.2, 200 mM Sodium chloride |
