8PXU
Targeting extended blood antigens by Akkermansia muciniphila enzymes unveils a missing link for generating universal donor blood
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 96.707, 170.738, 172.368 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.400 - 1.990 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.21880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.771 |
Data reduction software | xia2 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.400 | 2.061 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.148 | |
Rmeas | 0.153 | |
Rpim | 0.041 | 0.841 |
Number of reflections | 194025 | 268863 |
<I/σ(I)> | 9.65 | 0.56 |
Completeness [%] | 99.3 | |
Redundancy | 14 | |
CC(1/2) | 0.995 | 0.428 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 11% PEG8000, 0.5 M NaCl, 0.1M NaCacodylate |