8PXU

Targeting extended blood antigens by Akkermansia muciniphila enzymes unveils a missing link for generating universal donor blood

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	96.707, 170.738, 172.368
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.400 - 1.990
R-factor	0.19
Rwork	0.189
R-free	0.21880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.771
Data reduction software	xia2
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	2.061
High resolution limit [Å]	1.990	1.990
Rmerge	0.148
Rmeas	0.153
Rpim	0.041	0.841
Number of reflections	194025	268863
<I/σ(I)>	9.65	0.56
Completeness [%]	99.3
Redundancy	14
CC(1/2)	0.995	0.428

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	11% PEG8000, 0.5 M NaCl, 0.1M NaCacodylate