8PXG
Structure of Streptactin, solved at wavelength 2.75 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-09-06 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 2.7552 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 57.420, 57.420, 172.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.470 - 1.800 |
| R-factor | 0.17462 |
| Rwork | 0.174 |
| R-free | 0.19375 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.470 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.092 | 0.993 |
| Rmeas | 0.092 | |
| Rpim | 0.013 | |
| Number of reflections | 13199 | 1190 |
| <I/σ(I)> | 35.1 | 3.29 |
| Completeness [%] | 95.3 | |
| Redundancy | 45.6 | |
| CC(1/2) | 1.000 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 3 M sodium chloride, 0.1 citric acid, pH 3.5 |
