8PXF

Crystal structure of the full length Hh1412 Dsb homodimer.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 41 21 2
Unit cell lengths	87.260, 87.260, 130.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.630 - 2.130
R-factor	0.2318
Rwork	0.230
R-free	0.26410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.566
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.840	44.840	2.260
High resolution limit [Å]	2.130	6.330	2.130
Rmerge	0.120	0.108	1.814
Rmeas	0.126	0.114	1.895
Number of reflections	28892	1234	4564
<I/σ(I)>	9.58		1.01
Completeness [%]	100.0		100
Redundancy	11.45		11.97
CC(1/2)	0.993	0.988	0.527

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	Morpheus A4; 0.06 M Divalents, 0.1 M Buffer System 1 6.5, 50 % v/v Precipitant Mix 4