8PXF
Crystal structure of the full length Hh1412 Dsb homodimer.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 87.260, 87.260, 130.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.630 - 2.130 |
R-factor | 0.2318 |
Rwork | 0.230 |
R-free | 0.26410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.566 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.840 | 44.840 | 2.260 |
High resolution limit [Å] | 2.130 | 6.330 | 2.130 |
Rmerge | 0.120 | 0.108 | 1.814 |
Rmeas | 0.126 | 0.114 | 1.895 |
Number of reflections | 28892 | 1234 | 4564 |
<I/σ(I)> | 9.58 | 1.01 | |
Completeness [%] | 100.0 | 100 | |
Redundancy | 11.45 | 11.97 | |
CC(1/2) | 0.993 | 0.988 | 0.527 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | Morpheus A4; 0.06 M Divalents, 0.1 M Buffer System 1 6.5, 50 % v/v Precipitant Mix 4 |