8PXC
Structure of Fap1, a domain of the accessory Sec-dependent serine-rich glycoprotein adhesin from Streptococcus oralis, solved at wavelength 3.06 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I23 |
Synchrotron site | Diamond |
Beamline | I23 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-05-25 |
Detector | DECTRIS PILATUS 12M |
Wavelength(s) | 3.066 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.810, 30.400, 90.600 |
Unit cell angles | 90.00, 127.04, 90.00 |
Refinement procedure
Resolution | 65.530 - 1.973 |
R-factor | 0.18316 |
Rwork | 0.181 |
R-free | 0.22042 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.499 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | HKL2Map |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.530 | 2.043 |
High resolution limit [Å] | 1.973 | 1.973 |
Rmerge | 0.112 | |
Rmeas | 0.117 | |
Rpim | 0.031 | |
Number of reflections | 17626 | 1321 |
<I/σ(I)> | 20.29 | 8.67 |
Completeness [%] | 84.3 | |
Redundancy | 12.6 | |
CC(1/2) | 0.990 | 0.962 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M BIS-Tris 5.5 25 % w/v PEG 3350 |