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8PX5

Structure of the RNA recognition motif (RRM) of Seb1 from S. pombe., solved at wavelength 2.75 A

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I23
Synchrotron siteDiamond
BeamlineI23
Temperature [K]80
Detector technologyPIXEL
Collection date2016-07-04
DetectorDECTRIS PILATUS 12M
Wavelength(s)2.7552
Spacegroup nameC 1 2 1
Unit cell lengths111.100, 46.900, 32.300
Unit cell angles90.00, 99.00, 90.00
Refinement procedure
Resolution54.870 - 1.770
R-factor0.15652
Rwork0.155
R-free0.18829
Structure solution methodSAD
RMSD bond length0.010
RMSD bond angle1.094
Data reduction softwareXDS
Data scaling softwareAimless
Phasing softwareHKL2Map
Refinement softwareREFMAC (5.8.0238)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]54.8701.838
High resolution limit [Å]1.7751.775
Rmerge0.0450.252
Rmeas0.049
Rpim0.019
Number of reflections10445653
<I/σ(I)>22.913.26
Completeness [%]64.9
Redundancy6.1
CC(1/2)0.9990.919
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP2931 M ammonium formate, 100 mM sodium cacodylate, 8% (w/v) poly-gamma-glutamic acid polymer (PGA-LM, 200-400 kDa low molecular weight polymer)

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