8PX5

Structure of the RNA recognition motif (RRM) of Seb1 from S. pombe., solved at wavelength 2.75 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I23
Synchrotron site	Diamond
Beamline	I23
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-07-04
Detector	DECTRIS PILATUS 12M
Wavelength(s)	2.7552
Spacegroup name	C 1 2 1
Unit cell lengths	111.100, 46.900, 32.300
Unit cell angles	90.00, 99.00, 90.00

Refinement procedure

Resolution	54.870 - 1.770
R-factor	0.15652
Rwork	0.155
R-free	0.18829
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.094
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	HKL2Map
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.870	1.838
High resolution limit [Å]	1.775	1.775
Rmerge	0.045	0.252
Rmeas	0.049
Rpim	0.019
Number of reflections	10445	653
<I/σ(I)>	22.91	3.26
Completeness [%]	64.9
Redundancy	6.1
CC(1/2)	0.999	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1 M ammonium formate, 100 mM sodium cacodylate, 8% (w/v) poly-gamma-glutamic acid polymer (PGA-LM, 200-400 kDa low molecular weight polymer)