8PX0
Structure of ribonuclease A, solved at wavelength 2.75 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-08-01 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 2.7552 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.980, 32.620, 72.490 |
| Unit cell angles | 90.00, 90.16, 90.00 |
Refinement procedure
| Resolution | 50.010 - 1.800 |
| R-factor | 0.20252 |
| Rwork | 0.201 |
| R-free | 0.23851 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.420 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.093 | 0.341 |
| Rmeas | 0.095 | 0.364 |
| Rpim | 0.021 | 0.120 |
| Number of reflections | 17977 | 1412 |
| <I/σ(I)> | 24.67 | 5.03 |
| Completeness [%] | 81.3 | 65.04 |
| Redundancy | 17.5 | 8.2 |
| CC(1/2) | 0.995 | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20 mM sodium citrate pH 5.5, 28 % PEG 4000 |
