8PWV
PfRH5 bound to monoclonal antibody MAD8-502
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-24 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.95374 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.970, 158.011, 113.017 |
| Unit cell angles | 90.00, 94.59, 90.00 |
Refinement procedure
| Resolution | 79.000 - 2.070 |
| R-factor | 0.2692 |
| Rwork | 0.269 |
| R-free | 0.27910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (20-OCT-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.010 | 2.110 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Number of reflections | 181986 | 8654 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.997 | 0.370 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.04 M potassium phosphate monobasic, 16% w/v PEG 8000, 20% w/v glycerol |
