8PWU
PfRH5 bound to monoclonal antibody MAD10-255
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-24 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.95374 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 115.309, 134.522, 198.667 |
| Unit cell angles | 90.00, 93.56, 90.00 |
Refinement procedure
| Resolution | 87.450 - 3.148 |
| R-factor | 0.2431 |
| Rwork | 0.242 |
| R-free | 0.26360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.030 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (20-OCT-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.450 | 3.200 |
| High resolution limit [Å] | 3.148 | 3.150 |
| Number of reflections | 104997 | 5215 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7 | |
| CC(1/2) | 0.996 | 0.264 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M ammonium sulphate, 0.3 M sodium formate, 0.1 M Tris pH 7.8, 2% w/v g-PGA (Na+ form, LM), 3% w/v PEG 20000 |
