8PW2

Protein p6 from bacteriophage phi29, C-terminal delta31 truncated version

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 31 2 1
Unit cell lengths	59.257, 59.257, 41.668
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.350 - 1.590
R-factor	0.2128
Rwork	0.213
R-free	0.21630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.076
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.670	1.620
High resolution limit [Å]	1.590	1.590
Rmerge		1.026
Number of reflections	11565	550
<I/σ(I)>	37.3
Completeness [%]	99.3
Redundancy	19.5
CC(1/2)	1.000	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	100 mM Bis Tris Propane pH 7.0 and 1.3 M di-Ammonium Tartrate