8PW0

Manganese-dependent transcriptional repressor DR2539 complexed with manganese and bound to dr1709 promotor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976250
Spacegroup name	P 42 21 2
Unit cell lengths	67.710, 67.710, 158.137
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.880 - 2.200
R-factor	0.1849
Rwork	0.183
R-free	0.22730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.095
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.880	2.320
High resolution limit [Å]	2.200	2.200
Number of reflections	19507	2768
<I/σ(I)>	13.1
Completeness [%]	100.0
Redundancy	8.8
CC(1/2)	0.998	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 8K 26% (w/v); Imidazole 0.1 M pH 7.8; NaCl 0.2 M