8PVS

Targeting extended blood antigens by Akkermansia muciniphila enzymes unveils a missing link for generating universal donor blood

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976300
Spacegroup name	C 2 2 21
Unit cell lengths	118.527, 134.035, 215.346
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.990 - 1.680
R-factor	0.1713
Rwork	0.170
R-free	0.19980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.937
Data reduction software	xia2
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.990	1.740
High resolution limit [Å]	1.680	1.680
Rmerge	0.168
Rmeas	0.174
Rpim	0.046
Number of reflections	193688	19152
<I/σ(I)>	10.01	0.44
Completeness [%]	99.9	99.29
Redundancy	13.9
CC(1/2)	0.999	0.346

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.9	291	8% PEG20.000, 8% PEG550MME, 0.02 M Tris (pH 8.9), 1.2 M NaFormate