8PVS
Targeting extended blood antigens by Akkermansia muciniphila enzymes unveils a missing link for generating universal donor blood
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976300 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 118.527, 134.035, 215.346 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.990 - 1.680 |
| R-factor | 0.1713 |
| Rwork | 0.170 |
| R-free | 0.19980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.937 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.990 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.168 | |
| Rmeas | 0.174 | |
| Rpim | 0.046 | |
| Number of reflections | 193688 | 19152 |
| <I/σ(I)> | 10.01 | 0.44 |
| Completeness [%] | 99.9 | 99.29 |
| Redundancy | 13.9 | |
| CC(1/2) | 0.999 | 0.346 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.9 | 291 | 8% PEG20.000, 8% PEG550MME, 0.02 M Tris (pH 8.9), 1.2 M NaFormate |
