8PUZ

Crystal structure of tropomyosin (Cdc8) cables, Conformer 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	23.267, 38.616, 98.933
Unit cell angles	94.31, 91.92, 102.94

Refinement procedure

Resolution	24.630 - 2.200
R-factor	0.2352
Rwork	0.233
R-free	0.28030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.320
Data reduction software	SADABS
Data scaling software	SADABS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.600	2.340
High resolution limit [Å]	2.200	2.200
Number of reflections	16997	2706
<I/σ(I)>	10.5
Completeness [%]	99.9
Redundancy	2.1
CC(1/2)	0.915	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	1 M Tris HCl pH 8.2, and 45% MPD