8PUU

Giardia intestinalis deoxyadenosine kinase forms a functional tetramer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976254
Spacegroup name	P 32 2 1
Unit cell lengths	103.130, 103.130, 147.109
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.040 - 2.100
Rwork	0.200
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS (VERSION Jan 10, 2022 BUILT=20220820)
Data scaling software	Aimless (0.7.9)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.040	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.092
Rmeas	0.097
Rpim	0.030
Number of reflections	53424	5191
<I/σ(I)>	15.4	2.6
Completeness [%]	99.7	99.12
Redundancy	20
CC(1/2)	0.999	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294.15	Morpheus Screen condition H3: 0.1 M amino acids, 0.1 M Buffer 1 pH 6.5, 30% GOL_P4K