8PU3

Complex of the toxin/antitoxin FaRel2/ATfaRel2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	90
Detector technology	PIXEL
Collection date	2021-07-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	51.957, 106.231, 135.105
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.550 - 2.170
R-factor	0.1869
Rwork	0.184
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.492
Refinement software	BUSTER (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.400	3.360
High resolution limit [Å]	2.140	2.140
Number of reflections	26913	1346
<I/σ(I)>	11.3
Completeness [%]	94.1
Redundancy	39.9
CC(1/2)	0.990	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	35% v/v 1,4-Dioxane