8PTE
Polyoxidovanadate interaction with proteins: crystal structure of lysozyme bound to octadecavanadate ion (structure B)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 80.390, 80.390, 36.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.128 - 1.797 |
| Rwork | 0.180 |
| R-free | 0.23250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.071 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.190 | 1.830 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.104 | 1.060 |
| Number of reflections | 11611 | 576 |
| <I/σ(I)> | 33.6 | 4.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 20 | 15 |
| CC(1/2) | 1.000 | 0.912 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 20% ethylene glycol, 0.1 M sodium acetate buffer pH 4.0, 0.6 M sodium nitrate |
