8PS6
Crystal structure of the N-terminal domain of SduA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-21 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.99999 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 225.543, 225.543, 32.029 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.620 - 2.520 |
| R-factor | 0.2235 |
| Rwork | 0.222 |
| R-free | 0.24450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.195 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.772 | 2.561 |
| High resolution limit [Å] | 2.518 | 2.518 |
| Rmerge | 0.174 | |
| Rmeas | 0.178 | |
| Rpim | 0.039 | |
| Number of reflections | 10123 | 518 |
| <I/σ(I)> | 14 | |
| Completeness [%] | 95.8 | 100 |
| Redundancy | 20.3 | 21.5 |
| CC(1/2) | 0.748 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277.15 | HEPES 100 mM, Ammonium Sulfate 550 mM, protein concentration: 4.5 mg /ml, 1 uL reservoir + 1 uL protein solution. |
