8PRK
THE R78K AND D117E ACTIVE SITE VARIANTS OF SACCHAROMYCES CEREVISIAE SOLUBLE INORGANIC PYROPHOSPHATASE: STRUCTURAL STUDIES AND MECHANISTIC IMPLICATIONS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-09-01 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.700, 102.300, 117.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.850 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wgj |
RMSD bond length | 0.010 |
RMSD bond angle | 25.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.062 | |
Total number of observations | 524728 * | |
Number of reflections | 54387 | |
<I/σ(I)> | 10.8 | |
Completeness [%] | 89.3 | 92.5 |
Redundancy | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6 | 4 * | 17-19 % MPD 25 MM MES, PH 6.0 1 MM MNCL2 0.5 MM NA2HPO4 10 MG/ML PROTEIN |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | MES | 25 (mM) | |
3 | 1 | drop | 1 (mM) | ||
4 | 1 | drop | sodium hydrogen phosphate | 0.5 (mM) | |
5 | 1 | drop | MPD | 17 (%(v/v)) | |
6 | 1 | reservoir | MPD | 19 (%(v/v)) |