8PQJ
PDGFRA wild-type kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-14 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9998 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.380, 49.180, 77.040 |
| Unit cell angles | 90.00, 100.78, 90.00 |
Refinement procedure
| Resolution | 37.840 - 1.820 |
| R-factor | 0.1854 |
| Rwork | 0.184 |
| R-free | 0.21510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.776 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.340 | 1.900 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmeas | 0.087 | 1.713 |
| Number of reflections | 32011 | 3827 |
| <I/σ(I)> | 12.45 | 1.21 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.84 | 6.52 |
| CC(1/2) | 0.999 | 0.461 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285.15 | 10 mg/mL, 15% PEG3350, 100 mM Bis-Tris-Propane, pH 7.5 |
