8PQ4

Structure of HosA transcriptional regulator from enteropathogenic Escherichia coli O127:H6 (strain E2348/69)

Replaces:  7Z4P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-12-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54179
Spacegroup name	P 43 2 2
Unit cell lengths	67.160, 67.160, 95.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.490 - 2.250
R-factor	0.2144
Rwork	0.212
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.482
Data reduction software	iMOSFLM (7.4.0)
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX ((1.19.2_4158))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.970	2.330
High resolution limit [Å]	2.210	2.210
Rmerge	0.140	0.760
Rmeas	0.152	0.827
Rpim	0.058	0.321
Number of reflections	11402	1524
<I/σ(I)>	9.3	2.3
Completeness [%]	99.0	93.3
Redundancy	6.7	6.4
CC(1/2)	0.996	0.669

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.6	277.15	Ammonium acetate, Bis Tris HCl, PEG 3350