8POQ

Crystal structure of wolbachia leucyl-tRNA synthetase editing domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97549
Spacegroup name	P 43
Unit cell lengths	53.640, 53.640, 267.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.780 - 3.000
R-factor	0.2787
Rwork	0.276
R-free	0.32630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.548
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.780	3.100
High resolution limit [Å]	2.850	2.850
Rmeas	0.050	2.190
Number of reflections	14906	739
<I/σ(I)>	16.6	1
Completeness [%]	99.9	99.6
Redundancy	6.8	6.8
CC(1/2)	0.990	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M Tris pH8.5; 1 M LiCl2; 18 % w/v PEG 6000