8POO
Low resolution structure of inactive conformation of the Ktr cation channel in presence of ATP and c-di-AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-15 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98013 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 127.795, 143.686, 160.669 |
Unit cell angles | 90.00, 112.37, 90.00 |
Refinement procedure
Resolution | 48.000 - 5.770 |
R-factor | 0.4069 |
Rwork | 0.405 |
R-free | 0.42560 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.635 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.530 | 6.450 |
High resolution limit [Å] | 5.770 | 5.770 |
Rmerge | 0.084 | 1.359 |
Rmeas | 0.091 | 1.472 |
Rpim | 0.035 | 0.561 |
Number of reflections | 15151 | 4157 |
<I/σ(I)> | 9.6 | 1.4 |
Completeness [%] | 98.6 | 95.7 |
Redundancy | 6.9 | 6.8 |
CC(1/2) | 0.998 | 0.687 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Tris-HCl pH 8.5 700 mM KCl 24% PEG 400 |