8POF

The crystal structure of RsSymEG1 reveals a unique form of smaller GH7 endoglucanases alongside GH7 cellobiohydrolases in protist symbionts of termites

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	I 41
Unit cell lengths	114.604, 114.604, 49.963
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.800 - 1.850
Rwork	0.166
R-free	0.18660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.226
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.800	1.890
High resolution limit [Å]	1.850	1.850
Number of reflections	27804	1666
<I/σ(I)>	9.8	2.6
Completeness [%]	99.8
Redundancy	4.2
CC(1/2)	0.996	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.03 M Sodium fluoride, 0.03 M Sodium bromide, 0.03 M Sodium iodide, 12% v/v PEG 500 MME, 6 % w/v PEG 20000, 0.1 M Tris-Bicine pH 8.5