8POB

Crystal structure of Hen Egg White Lysozyme co-crystallized with 10 mM TbXo4-SO3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.648
Spacegroup name	P 43 21 2
Unit cell lengths	77.404, 77.404, 37.602
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.700 - 1.740
R-factor	0.1827
Rwork	0.180
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.291
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.700	1.840
High resolution limit [Å]	1.740	1.740
Rmerge	0.074	0.855
Rpim	0.028	0.184
Number of reflections	12161	1719
<I/σ(I)>	0.248	0.04
Completeness [%]	99.9
Redundancy	24.1
CC(1/2)	0.998	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	950 mM sodium chloride, 100mM sodium acetate pH=4.6. Drops were set by mixing one volume of protein solution at 20mg/ml with one volume of 10mM TbXo4 and with one volume of the well solution.