8PO6
Structure of Escherichia coli HrpA apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978520 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.605, 114.897, 94.011 |
Unit cell angles | 90.00, 100.86, 90.00 |
Refinement procedure
Resolution | 29.730 - 2.660 |
R-factor | 0.2086 |
Rwork | 0.206 |
R-free | 0.25740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.603 |
Data reduction software | SORTAV (BUILT 20190315) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.17rc5_3630) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.328 | 2.702 |
High resolution limit [Å] | 2.656 | 2.656 |
Rmerge | 0.082 | 0.587 |
Rmeas | 0.090 | 0.669 |
Rpim | 0.035 | 0.314 |
Number of reflections | 23640 | 1051 |
<I/σ(I)> | 14.6 | 2 |
Completeness [%] | 99.0 | 88.8 |
Redundancy | 6.3 | 4.3 |
CC(1/2) | 0.998 | 0.891 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1M Hepes 7% PEG 20000 |