8PNY
Crystal structure of D-amino acid aminotransferase from Blastococcus saxobsidens complexed with phenylhydrazine and in its apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-31 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 104.644, 104.644, 51.541 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.590 - 1.800 |
| R-factor | 0.20568 |
| Rwork | 0.203 |
| R-free | 0.25406 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.790 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.590 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.215 | 1.520 |
| Rmeas | 0.224 | 1.583 |
| Rpim | 0.062 | 0.437 |
| Total number of observations | 389046 | 22104 |
| Number of reflections | 30251 | 1727 |
| <I/σ(I)> | 10.7 | 1.9 |
| Completeness [%] | 99.6 | |
| Redundancy | 12.9 | 12.8 |
| CC(1/2) | 0.996 | 0.506 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.1 M Hepes pH 7.5, 4 M NaCl |
