8PNR

Structure of human KCTD15 BTB domain mutant G88D crystal form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.976254
Spacegroup name	P 43 21 2
Unit cell lengths	151.081, 151.081, 155.987
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.750 - 2.250
R-factor	0.2481
Rwork	0.246
R-free	0.28540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.246
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.990	2.290
High resolution limit [Å]	2.250	2.250
Number of reflections	85815	4227
<I/σ(I)>	14.8
Completeness [%]	100.0
Redundancy	27.28
CC(1/2)	0.995	0.313

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	17.5% PEG3350, 0.3M Potassium thiocyanate