8PNK
Crystal structure of the Ded1p RecA1 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-25 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.000036 |
Spacegroup name | P 61 |
Unit cell lengths | 110.000, 110.000, 52.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.630 - 2.200 |
R-factor | 0.1908 |
Rwork | 0.189 |
R-free | 0.22460 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.541 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.630 | 2.279 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.049 | 0.712 |
Rmeas | 0.053 | 0.781 |
Rpim | 0.020 | 0.318 |
Number of reflections | 18435 | 1764 |
<I/σ(I)> | 21.69 | 2.48 |
Completeness [%] | 99.7 | 97.4 |
Redundancy | 6.6 | 5.8 |
CC(1/2) | 1.000 | 0.778 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M HEPES pH 7.0, 30% Jeffamine ED-2001 pH 7.0 |