8PNI
Chorismate mutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-17 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 32 |
| Unit cell lengths | 48.445, 48.445, 113.364 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.350 - 1.210 |
| Rwork | 0.143 |
| R-free | 0.17060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.472 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0350) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.350 | 1.230 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Number of reflections | 88399 | 3688 |
| <I/σ(I)> | 15 | 1.5 |
| Completeness [%] | 97.4 | 79.8 |
| Redundancy | 9.8 | 7.8 |
| CC(1/2) | 0.999 | 0.507 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 1:3 volume ratio of 8 mg/mL protein in 20 mM Bis-Tris, pH 6.5 storage buffer and 0.2 M ammonium citrate dibasic 20% (w/v) PEG 3350 |
