8PNC
Transcription factor BARHL2 bound to TAAGT DNA sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.519, 123.711, 62.365 |
| Unit cell angles | 90.00, 93.94, 90.00 |
Refinement procedure
| Resolution | 39.460 - 2.050 |
| R-factor | 0.2337 |
| Rwork | 0.232 |
| R-free | 0.26720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.397 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.870 | 43.870 | 2.110 |
| High resolution limit [Å] | 2.050 | 8.930 | 2.050 |
| Rmerge | 0.170 | 0.049 | 2.305 |
| Rmeas | 0.190 | 0.055 | 2.599 |
| Rpim | 0.083 | 0.023 | 1.176 |
| Total number of observations | 194035 | 2389 | 13664 |
| Number of reflections | 37375 | 469 | 2846 |
| <I/σ(I)> | 7.7 | 28.1 | 0.8 |
| Completeness [%] | 99.6 | 98.6 | 97.1 |
| Redundancy | 5.2 | 5.1 | 4.8 |
| CC(1/2) | 0.993 | 0.994 | 0.188 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | PEG 4000, sodium malonate, sodium acetate |
