8PN0
HEV gt3 P domain in complex with glycan-sensitive nAb p60.12
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.430, 94.480, 91.250 |
| Unit cell angles | 90.00, 90.21, 90.00 |
Refinement procedure
| Resolution | 47.240 - 2.072 |
| R-factor | 0.2276 |
| Rwork | 0.226 |
| R-free | 0.26080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.240 | 2.146 |
| High resolution limit [Å] | 2.072 | 2.072 |
| Rmeas | 0.129 | |
| Number of reflections | 77331 | 3527 |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 84.8 | |
| Redundancy | 3.27 | |
| CC(1/2) | 0.991 | 0.193 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 25% w/v PEG 4000, 0.2M Calcium chloride and 0.1M Tris pH 8.5 |
