8PMW

HEV gt3 P domain in complex with glycan-sensitive nAb p60.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-17
Detector	DECTRIS PILATUS 12M
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	75.810, 159.260, 205.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.440 - 1.980
R-factor	0.2073
Rwork	0.206
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.440	2.051
High resolution limit [Å]	1.980	1.980
Rmeas	0.130
Number of reflections	86313	8192
<I/σ(I)>	12.43
Completeness [%]	99.5
Redundancy	13.23
CC(1/2)	0.998	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	2M (NH4)2SO4 and 0.1M sodium acetate pH 4.6