8PMU

Crystal structure of the HC7 apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 41 21 2
Unit cell lengths	93.854, 93.854, 87.287
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.360 - 1.750
R-factor	0.19293
Rwork	0.191
R-free	0.23117
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.932
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.850	1.840
High resolution limit [Å]	1.750	1.750
Rmerge	0.034	1.209
Number of reflections	36731	5728
<I/σ(I)>	11.5
Completeness [%]	91.9
Redundancy	8.7
CC(1/2)		0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Lithium chloride Tris PEG 8000