8PMU
Crystal structure of the HC7 apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.854, 93.854, 87.287 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.360 - 1.750 |
| R-factor | 0.19293 |
| Rwork | 0.191 |
| R-free | 0.23117 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.932 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.850 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.034 | 1.209 |
| Number of reflections | 36731 | 5728 |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 91.9 | |
| Redundancy | 8.7 | |
| CC(1/2) | 0.644 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | Lithium chloride Tris PEG 8000 |
