8PMF
transcription factor BARHL2 bound to DNA sequences
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.61992 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.857, 47.286, 72.557 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.620 - 0.950 |
| R-factor | 0.1453 |
| Rwork | 0.144 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.917 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.290 | 47.290 | 0.970 |
| High resolution limit [Å] | 0.950 | 5.130 | 0.950 |
| Rmerge | 0.049 | 0.044 | 3.237 |
| Rmeas | 0.053 | 0.049 | 3.544 |
| Rpim | 0.021 | 0.021 | 1.421 |
| Total number of observations | 548989 | 3286 | 24814 |
| Number of reflections | 84114 | 627 | 4170 |
| <I/σ(I)> | 15.3 | 48.5 | 0.5 |
| Completeness [%] | 99.7 | 98.1 | 96.9 |
| Redundancy | 6.5 | 5.2 | 6 |
| CC(1/2) | 0.998 | 0.989 | 0.169 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | PEG 4000, sodium malonate, sodium acetate |
