8PM7
transcription factor BARHL2 bound to TAATC DNA sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.627, 123.807, 62.713 |
| Unit cell angles | 90.00, 93.53, 90.00 |
Refinement procedure
| Resolution | 44.050 - 1.700 |
| R-factor | 0.2084 |
| Rwork | 0.207 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.481 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.050 | 44.010 | 1.730 |
| High resolution limit [Å] | 1.700 | 8.980 | 1.700 |
| Rmerge | 0.057 | 0.031 | 2.133 |
| Rmeas | 0.066 | 0.036 | 2.571 |
| Rpim | 0.034 | 0.019 | 1.411 |
| Total number of observations | 1604 | 9309 | |
| Number of reflections | 65847 | 454 | 3163 |
| <I/σ(I)> | 9.5 | 35.3 | 0.5 |
| Completeness [%] | 99.1 | 96.4 | 88.9 |
| Redundancy | 3.8 | 3.5 | 2.9 |
| CC(1/2) | 0.999 | 0.997 | 0.297 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | PEG 4000, sodium malonate, sodium acetate |
