8PM1
Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) variant I52V A85Q
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-05-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 53.817, 110.431, 39.314 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.215 - 1.500 |
Rwork | 0.197 |
R-free | 0.22850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.632 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 110.430 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 37959 | 1833 |
<I/σ(I)> | 8.5 | 0.7 |
Completeness [%] | 98.9 | |
Redundancy | 14 | |
CC(1/2) | 0.998 | 0.447 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Calcium acetate hydrate 0.1 M Sodium cacodylate trihydrate pH 6.5 18% w/v Polyethylene glycol 8,000 |